单克隆抗体生产的杂交瘤技术_杂交瘤制备单克隆抗体

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Hybridoma Technology for the Generation of Monoclonal Antibodies(mAbs)单克隆抗体生成的杂交瘤技术 Abstract 1 Introduction 2 Materials

2.1 Preparation of splenocytes 2.1 脾细胞制备spleens from immunized mice 免疫小鼠的脾脏RPMI-1640 medium(Serum-free cell freezing medium)无血清细胞冻存培养基 无血清细胞冻存培养基 3 Petri dishes 有盖培养皿Sterile surgical instruments,including microdiecting sciors and forceps,for collecting animal samples 用于收集动物样品的无菌手术器械，包括显微解剖剪和钳子，5 sterile microscope gla slide with frosted ends 磨砂的的无菌显微玻片 6 15-ml conical tubes 15-ml 锥形瓶

2.2 Preparation of myeloma cells as the fusion partner 作为融合头（融合标签）的骨髓瘤细胞的制备 2.3 cell fusion 细胞融合2.4 hybridoma screening by FACS 2.5 hybridoma screening by ELISA 通过ELISA进行杂交瘤筛选 2.6 hybridoma screening by IHC 2.7 hybridoma subcloning 2.8 hybridoma cryopreservation 低温保存杂交瘤细胞 2.9 antibody isotyping 抗体同型

2.10 thawing and growth of hybridoma cells 杂交瘤细胞的融化和生长methods 3.1 preparation of splenocytes from the immunized mouse 来自免疫鼠的脾细胞的制备 3.2 preparation of myeloma cells 骨髓瘤细胞的制备 3.3 cell fusion 细胞融合3.4 hybridoma screening using flow cytometry 通过流式细胞仪进行杂交瘤筛选 3.5 hybridoma screening by ELISA 通过ELISA进行杂交瘤筛选 3.6 hybridoma screening by IHC 通过免疫组化进行杂交瘤筛选 3.7 hybridoma subcloning 杂交瘤亚克隆

3.8 hybridoma cryopreservation 杂交瘤低温保存 3.9 antibody isotyping 抗体同型

3.10 Thawing and growth of hybridoma cells 杂交瘤细胞的融化和生长 4 小记 参考文献