ScU诱导培养基_诱导培养基

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Sc－U诱导培养基

SC Minimal Medium and Plates

SC is synthetic minimal defined medium for yeast.0.67% yeast nitrogen base(without amino acids)

2% carbon source(i.e.glucose or raffinose棉子糖)

0.01%(adenine, arginine, cysteine, leuine, lysine, threonine, tryptophan, uracil)

0.005%(aspartic acid, histidine, isoleucine, methionine, phenylalanine, proline, serine, tyrosine, valine)

2% agar(for plates)

1.Diolve the following reagents in 900 ml deionized water(800 ml if preparing medium containing raffinose).Note: We make medium and plates as we need them and weighout each amino acid.Many researchers prepare 100X solutions of each amino acid that they need.Reminder: Omit uracil to make selective plates for growing pYES2 transformants.6.7 g Yeast Nitrogen Base

0.1 g each 0.05 g each

adenine aspartic acid

arginine histidine

cysteine isoleucine

leucine methionine

lysine phenylalanine

threonine proline

tryptophan serine

uracil(U)tyrosine

valine

2.If you are making plates, add the agar after diolving the reagents above.3.Autoclave at 15 psi, 121°C for 20 minutes.4.Cool to 50°C and add 100 ml of filter-sterilized 20% glucose or 200 ml of filtersterilized10% raffinose.5.Pour plates and allow to harden.Invert the plates and store at +4°C.Plates are stable for 6 months.Induction Medium

If you are making induction medium, follow Steps 1-3 above except diolve the reagents in 800 ml of deionized water.Cool the medium to 50°C and add 100 ml of filter-sterilized 20% galactose and 100 ml of filter-sterilized 10% raffinose to the medium.