利用丙酮丁醇发酵废水偶联乙醇发酵的研究_丙酮丁醇发酵
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利用丙酮丁醇发酵废水偶联乙醇发酵的研究*
左文朴1 裴建新1 庞浩
1黄志民1 黎贞崇1
韦宇拓2 黄日波1, 2
(1.广西科学院国家非粮生物质能源工程技术研究中心,广西 南宁 530003;2.微生物及植物遗传工程教育部重点实验室,广西 南宁 530005)
摘要:丙酮-丁醇发酵生产过程中产生大量废液,本研究以一株从自然环境中分离得到的酿酒酵母GXAS-BT9作为发酵菌株,利用丙酮-丁醇发酵的废液作为乙醇发酵的配浆用水,进行乙醇发酵。实验结果表明:GXAS-BT9菌株的乙醇发酵产率随着废液比例的升高而增加,即使使用100%废液作为配浆用水,玉米粉和木薯粉作为原料的的乙醇产率分别达到14.27%(v/v)和14.26%(v/v),比对照分别提高了14.7%和9.6%。本研究将丁醇发酵与乙醇发酵偶联起来,实现了水的循环利用,同时大大减少了污水的排放量。
【关键词】 丙酮-丁醇发酵 废水 乙醇发酵 偶联 中图分类号: 文献标识码:
文章编号:
Study of resuing the waste water of acetone-butanol fermentation by coupling alcoholic
fermentation Zuo Wen-pu1 Pei Jian-xin1 Pang Hao1 Huang Zhi-min1 Li Zhen-chong1 Wei Yu-tuo2
Huang Ri-bo1, 2
(1.National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Science, Nanning 530003, Guangxi;2.Key Laboratory of Microbial and Plant Genetic Engineering of Ministry of Education, Guangxi University, Nanning 530005, Guangxi)
Abstract: A lot of waste water was produced from Acetone-butanol fermentation, this research take use of strain GXAS-BT9, which was a Saccharomyces cerevisiae separated from the natural environment, as the ethanol fermented strain and the waste water from Acetone-butanol fermentation as fermentation water.The results show that the ethanol production rate increase with the increase of the proportion of wastewater, ethanol production rate increase 14.7% and 9.6% when using cornmeal and caava meal, reached 14.27%(v/v)and 14.26%(v/v)respectively.Coupling the Acetone-butanol fermentation and ethanol fermentation will solve the water recycling and reduce the sewage discharge greatly.Key words: AcetoneRediscovering a Renewable Fuel [J].BioWorld Europe,2006,:16-19.Zverlov V., Berezina O., Velikodvorskaya G., et al.Bacterial Acetone and Butanol Production by Industrial Fermentation in the Soviet Union: Use of Hydrolyzed Agricultural Waste for Biorefinery [J].Applied Microbiology and Biotechnology,2006,71(5):587-597.[3] [4] [5] [6] [7] [8] [9] [10] Knoshaug Eric, Zhang Min.Butanol Tolerance in a Selection of Microorganisms [J].Applied Biochemistry and Biotechnology,2008,Lee S.Y., Park J.H., Jang S.H., et al.Fermentative Butanol Production by Clostridia [J].Biotechnol Bioeng,2008,101(2):209-228.王凯军, 秦人伟.发酵工业废水处理[M].北京: 化学工业出版社, 2000.王宇新, 刘学选.光合细菌法综合处理丙酮-丁醇发酵废水 [J].水处理技术,1995,21(5):291-294.周忙扣, 赵传义.厌氧-好氧生物法治理酒精醪液 [J].江苏环境科技,2000,13(4):10-12.Keis S., Bennett C.F., Ward V.K., et al.Taxonomy and Phylogeny of Industrial Solvent-Producing Clostridia [J].Int J Syst Bacteriol,1995,45(4):693-705.陈陶声, 陆祖祺.发酵法丙酮和丁醇生产技术[M].北京: 化学工业出版社, 1991.Kurtzman C.P., Fell J.W.The Yeasts, a Taxonomic Study, 4th Ed.[J].Amsterdam: Elseviers Scientific,1998,35:165-168